Biology coursework enzymes conclusion
Effect of temperature on enzyme activity experiment
This is because the molecules of both substrate and enzyme have more energy, therefore they collide more often and produce more heat energy. Apparatus Limitations Firstly, there were limitations on the apparatus that I used. For example, the final average volumes of gas were as follows: Those enzymes identified now number more than Hopefully, with the new and repeated results, I will be able to analyse my results further and therefore evaluate them with more evidence than I had previously. Since pancreatic amylase is a digestive enzyme its optimum temperature would be boy temperature ie. Seung Soo Jason Lee Improving the Investigation: Error Impact Improvement Time discrepancy between the moment amylase is inserted into the cuvette and Logger Pro reading is started It could have allowed more time for amylase to break down starch in some trials than in others, causing differences in rate of reaction from trial to trail and increasing standard deviation There are a few ways to improve this error. This could have meant longer time for the sodium chloride to denature the enzyme, thus lowering its rate of reaction. From your graph of activity against temperature, determine the optimum temperature. Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time. Because the volume of oxygen dissolved in the reaction is not constant for all reactions, and less oxygen is dissolved in water at higher temperatures, this would have affected my results. I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate. Draw a new graph. Evaluation Overall, I believe my experiment went well and that I gained sufficient results because I repeated each concentration three times and investigated eight concentrations in total. Many enzymes require the presence of another ion or a molecule called a cofactor, in order to function. Figure 6.
However, some oxygen was displaced in the gas syringe and I had to solve this by subtracting this small amount from the volumes produced in each of the reactions. When the products have been released, the enzyme is ready to bind with a new substrate. Figure 6.
Also, I noticed if the barrel was wet, the syringe often got stuck for a short time before it recorded the volumes of gas.
For this, I wanted to see how many potato discs would be a good number to do the actual experiment.
This means that having the same surface area of yeast in each reaction is very important in ensuring a fair test because the number of molecules exposed to collisions must be the same. I believe that my results were also relatively reliable because as the concentration decreased the volume of oxygen produced also decreased.
Overall, however, this investigation was successful in terms of the accuracy of its results. Though in theory, this should be the trend, my results did not demonstrate this pattern. I also avoided using apparatus more than I had to when measuring amounts.
Some enzymes, such as the proteinases trypsin and pepsin, retain the names used before this nomenclature was adopted.
Effect of hydrogen peroxide concentration on catalase
Again, judging by the accuracy of my repeat results, I believe that this factor was not an issue. The temperature coefficient Q10 for an enzyme controlled reaction between 0 and 45oC is 2. To prevent this I had to dry out the barrel and syringe before commencing the procedure. However, the decrease in the rate of reaction was not exponential; rather, the relationship between NaCl concentration and average rate of reaction was pretty linear. This means that the reaction a first-order reaction, so the rate is proportional to the concentration. Time discrepancy in the amount of time sodium chloride was allowed to interact with amylase between each trial It allowed more time for the sodium chloride in some micro tubes to denature amylase than in other micro tubes, allowing the possibility for further decrease in the enzyme activity for amylase used in some trials compared to other trials. Concentration of Hydrogen Peroxide The different concentrations of hydrogen peroxide that I made could not have been exactly accurate because this would have meant that the volume of gas evolved would have increased in equal steps, which it did not. I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate. The accuracy of the results showed that this factor did not distort the results too much, and so a similar amount of substrate molecules were present in each reaction. Account for the decline in the slope after the optimal temperature is attained. This could have affected my results for several reasons. This means the amount of substrate could have been different for each repeat, even though I used the same concentration.
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